Bacteriuria screening by use of acridine orange-stained smears
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چکیده
منابع مشابه
Thermal Depolarization of Fluorescence from Polytene Chromosomes Stained with Acridine Orange
The degree of polarization of fluorescence from stretched Chironomus thummi polytene chromosomes, stained with low concentrations of acridine orange (AO), decreases with increasing temperature. The "half temperature" of this decrease (T((1/2)R)) is lower than the expected DNA thermal denaturation temperature (T(m)) by about 20 degrees C. T((1/2)R) is lowered as histone is removed from chromosom...
متن کاملCarotenoid pigments and photokilling by acridine orange.
It has long been known that certain organic dyes can photosensitize cells, leading to cellular damage or death. Recently, studies were performed on one such dye, Toluidine Blue, to determine the mechanism of its lethal photosensitization of bacteria (M. M. Mathews, J. Bacteriol. 85:322, 1963). These studies showed that the cellular site of the lethal action was the cytoplasmic membrane and its ...
متن کاملAcridine Orange Binding by Micrococcus Lysodeikticus.
Beers, Roland F., Jr. (Johns Hopkins University, Baltimore, Md). Acridine orange binding by Micrococcus lysodeikticus. J. Bacteriol. 88:1249-1256. 1964.-Micrococcus lysodeikticus cells bind acridine orange (AO) reversibly. The adsorption isotherm is consistent with a highly cooperative-type binding similar to that observed with polyadenylic acid. The cells exhibit a strong buffering action on t...
متن کاملNOR-associated heterochromatin of pepper chromo- somes stained with acridine orange
Acridine orange is a non-specific fluorescent dye which binds to DNA mainly by intercalating between base pairs. In human and animal cytogenetics this dye has been extensively utilized to show reverse banding pattern (RFA). Considering the absence of Gand R-bands in plant chromosomes, the use of acridine orange after pretreatment in Cbanding protocols has been applied for detecting heterochroma...
متن کاملCompetitive Binding of Atp and Acridine Orange by Muscle.
Introduction.-The lifetime of electronic singlet excitation in molecules is too short to allow the utilization of these excitational energies in biological systems.' Triplet excitations last longer, but the probabilities of their occurrence are small. Circumstances which increase the probability of these transitions may thus have biological importance. It has been shown' that dyes and certain o...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1985
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.21.4.513-516.1985